This is a simple study with interesting implications for evolutionary genomics and microarray analysis. Here is our paper and online supplemental material.
Assessing the Conservation of Mammalian Gene Expression Using High-density Exon Arrays. Xing Y*, Ouyang Z, Kapur K, Scott MP, Wong WH*. Molecular Biology and Evolution, 2007, In press.
Microarray data from multiple species have been used to study evolutionary constraints on gene expression. Expression measurements from conventional microarray platforms such as the 3' expression arrays are strongly affected by platform-dependent probe effects that may introduce apparent, but misleading discrepancies between species. In this manuscript, we assess the conservation of mammalian gene expression in adult tissues using data from a high-density exon array platform. The exon arrays have more than six million probes on a single array targeting all exons in a genome. We find that, unlike 3' array data, gene expression measurements from exon arrays reveal patterns of gene expression that are highly conserved between humans and mice in multiple tissues. Our analysis provides strong evidence for widespread stabilizing selection pressure on transcript abundance during mammalian evolution.
